Lice Killing Efficacy of Elimax® Pure Power

Earlier experements showed that Elimax® PurePower yielded 100% mortality in head lice, treated for 10 and 15 min, respectively. Oystershell laboratories further evaluated lice killing efficacy of Elimax® Pure Power lotion following a 1- and 5-min treatment period. For this purpose, an in vitro study has been set-up at Tecnalia, Spain (01/2020).

  • After 1 min: 80% mean mortality at 5 min post-treatment application, going to 73% at 15 min up to 180 min, and increasing to 93% at 18 hours after product application.

  • After 5 min: 100% mean mortality at 5 min and 15 min post-treatment application, being 97% the value remaining from 30 min to 18 hours after product application.

Material and methods

Test product

Elimax® Pure Power lotion – Batch: 2019F20A; Exp. date: 06/2021.

Negative control

Tap water served as negative control.

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Insects

Head lice of both sexes(late stage nymphs and adults) were collected from the heads of volunteers and
immediately conditioned at the adequate environmental parameters (252°C y 2 70% RH) up to the
beginning of the test, between 3-5 hours after collection. After being examined under the binocular
microscope to select on behalf of their viability the ones to be used in the assay.

Test method

Viable lice were transferred with great care and soft tweezers to the center of a petri dish lined with
moistened Whatman no 4 filter paper with 0,4ml tap water. Only those specimensthat moved from the
center to the edge of the dish were used, being discarded those that remained motionless.

Groups of 10 individuals were treated by complete immersion into the product for 1 min and 5 min,
respectively. Following incubation, lice were rinsed with tap water and incubated in complete darkness.
Three replicates were conducted for both test product and negative control. Vital signs of crawling
stages were recorded at 5, 15, 30, 60, 180 minutes and 18 hours post-product application.

Results

Results are shown in the tables below:

– IMMERSION TIME: 1 MINUTE –

TIME POST-APPLICATION % MORALITY (MEAN ± SD)
NEGATIVE CONTROL «ELIMAX® PURE POWER»
5 MIN 0 80±0
15 MIN 0 73±6
30 MIN 0 73±6
60 MIN 0 73±6
180 MIN 0 73±6
18 HOURS 30 93±6

– IMMERSION TIME: 5 MINUTES –

TIME POST-APPLICATION % MORALITY (MEAN ± SD)
NEGATIVE CONTROL «ELIMAX® PURE POWER»
5 MIN 0 100±0
15 MIN 0 100±0
30 MIN 0 97±6
60 MIN 0 97±6
180 MIN 0 97±6
18 HOURS 40 97±6

Summarized, the following results were obtained:

  • After 4 min: 80% mean mortality at 5 min post-treatment application, going to 73% at 45 min
    up to 180 min, and increasing to 93% at 18 hours after product application.

  • After 5 min: 100% mean mortality at 5 min and 15 min post-treatment application, being 97%
    the value remaining from 30 min to 48 hours after product application.

Conclusion

Previous in vitro studies already demonstrated high efficacy of Elimax Pure Power (100% mortality)
following a treatment period of 10 and 15 min, respectively. Further testing confirmed high efficacy
of Elimax® Pure Power, being active from 1 min and showing almost 100% mortality after a S-min
treatment period.